COLUMN WATCH
The Cleaning and Regeneration of Reversed-Phase HPLC Columns
Ronald E. Majors, Agilent Technologies, Wilmington Delaware, USA.
This month’s “Column Watch” looks at practical ways to return a contaminated column to — or close to — its original state. Ron Majors also discusses cleaning procedures for bonded-silica and other types of reversed-phase columns.
`Reversed-phase chromatography is by far the most widely used technique in high performance liquid chromatography (HPLC).1 It is popular because it is applicable to most non-polar analytes and to many ionizable and ionic compou
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